Abstract: Atrial natriuretic peptide (ANP) plays a vital role in vasodilation, Broncho relaxation, pulmonary permeability, and in augmenting allergic inflammation and asthma. Natriuretic peptide precursor A gene encodes for, and it has been shown to have a significant association with asthma. This study was conducted to analyze the association between theNPPA gene polymorphism at rs5065 and the presence of asthma amongst Jordanian population. Blood samples were collected from 2 groups of Jordanians: 106 hmatic patients and 117 gendermatched ast-individuals who are non asthmatics using caseontrol design. Age, gender, onset of asthma, cseverity, and smoking history were obtained from all in the study. Both groups were genotyped for ​NPPA A2238G using Polymerase Chain ReactionRestriction Fragment Length Polymorphism (PCRRFLP) analysis.Cases and controls were matched only for gender and they differed with regard to their smoking habit and age. No statistically significant difference in rs5065 among -genotype frequencies for NPPAasthmatic patients and controls patients were founds (P>0.05). Stratification of data by gender also, revealed no statically significant difference in genotype frequencies amongst asthmatic and healthy controls patients. Step wise logistic regression analysis revealed that irrespective of any other differences between asthmatic patients and controls, carriers of the genotype AA were 1.6 to 1.7 more likely to develop asthma as compared to AG or GG. The findings of the current study, strengthens the potentially important role of genetic polymorphism at rs5065 in the prediction of asthma development, however future studies should be conducted with larger sample size and better distribution of gender, age groups and smoking status between cases and controls to identify significant association. Keywords: Genetic polymorphism, NPPA gene, rs5065, asthma, Jordan.​​