Kamal Eldeen Ahmed Elmobasher

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Research Title :Effect of Natural Cross-Linkers on Shear Bond Strength to Dentin After Storage in Different Media

Authors : Kamal Eldeen A. Elmobsher(1) Maha A. Niazy(2) ,Inas T. Motawea(3) andMayada S. Sultan(4)

Abstract :Objectives: This study was designed to evaluate the effect of natural cross linkers (Propolis and Hesperidin) on shear bond strength (SBS) to dentin after storage in different media. Materials and Methods: Ninety extracted molars were used and divided into three main groups according to dentin surface treatment (n=30) where, A1: treated with Propolis, A2: treated with Hesperidin and A3: receive no treatment to serve as a control group. Each group was further subdivided into 3 subgroups (n=10) according to the storage media either stored in Acids (B1) or stored in Artificial saliva (B2) or stored in distilled Water (B3). The shear bond strength (SBS) was determined using a universal testing machine. Results: The dentin specimens treated with Propolis showed significantly highest SBS. Conclusion: Both Propolis and Hesperidin cross linking agents are considered beneficial in improving bonding to dentin, though Propolis was much more effective. Storage media has deteriorating effect on resin-dentin shear bond strength.

Keywords :Collagen cross-linkers, Propolis, Hesperidin, Shear bond strength

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Research Title :IN VIVO EVALUATION OF ELECTROPHORESIS-AIDED REMINERALIZATION ON MICROHARDNESS OF DEMINERALIZED ENAMEL

Authors : Kamal Eldeen Ahmed Elmobsher 1*, Sameh Mahmoud Nabih 2

Abstract :Objective: evaluate the electrophoresis effect on the micro-hardness of demineralized enamel in comparison to traditional remineralization using different remineralizing agents. Subjects and Methods: Initial enamel lesion was produced in rabbits with an acid etching, then, on a labial surface; three remineralizing agents were placed.; nano-hydroxyapatite (n-HAP), casein phosphor-peptide amorphous calcium phosphate (CPP-ACP), and bioactive glass (BAG) with traditional technique and electrophoresis technique. Surface micro-hardness using a Vickers test at baseline, demineralization, and after remineralization at different times was calculated. The normality of the data evaluated using the Shapiro-Wilk and Kolmogorov-Smirnov tests. P ≤ 0.05 was used to compare different variables using the ANOVA test and Tukey’s post-hoc analysis. Results: The enamel hardness was greatly raised by all three remineralizing agents utilized in the study following application periods. For the traditional method n-HAP shows the highest remineralizing effect with, the lowest impact revealed in BAG material after two and five weeks. Moreover, in electrophoresis n-HAP material revealed the highest remineralizing effect, followed by CPP-ACP material with the most insufficient effect shown in BAG material after three and five hours. Conclusion: the application of the used different remineralizing agents enhances enamel remineralization, has an influential effect on enamel surface hardness, extended time produces an additive effect in the remineralization process and electrophoresis can both strengthen and re-hardening effects on demineralized enamel, also expedite the remineralizing agents’ effect.

Keywords : Enamel remineralization, electrophoresis, microhardness. https://ajdsm.journals.ekb.eg/article_414469.html

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Research Title :Evaluation of Electrophoresis-Aided Remineralization On the Surface Morphology Of Demineralized Enamel

Authors :Kamal eldeen A. Elmobsher*1 Sameh M. Nabih**2 Hamed I. Elkady***3

Abstract :Aim: This in-vivo animal study aimed to evaluate whether an electrophoresis-aided system improved remineralization of demineralized enamel more than the traditional technique in rabbits with initial enamel lesions using different remineralizing agents. Materials and Methods: The initial enamel lesion was produced through acid etching, three remineralizing agents (nano-hydroxyapatite(n-HAP), casein phospho-peptide amorphous calcium phosphate (CPP-ACP), and bioactive glass (BAG)) were applied to the labial surface using traditional or electrophoresis-aided techniques. Surface topography was evaluated using ESEM at different periods. ESEM images were imported into Image J. software for further analysis. Results: ESEM showed that remineralizing agents enhanced remineralization of surface topography following different application periods. Conclusion: Applying different remineralizing agents significantly influences enamel topography. Further time has a significant positive impact on remineralization. Moreover, the dynamics of remineralizing agents might be accelerated by electrophoresis

Keywords : In Vivo animal model, enamel remineralization, electrophoresis, image J.

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Research Title :In Vivo Evaluation of Electrophoresis-Aided Remineralization On the Surface Topography and Chemical Composition Of Demineralized Enamel

Authors :

Abstract :Objectives: This in vivo animal study aimed to evaluate whether an electrophoresis-aided system would improve the remineralization of demineralized enamel more than the traditional technique in rabbits with initial enamel lesions using different remineralizing agents. Methods: Initial enamel lesion was produced following an acid etching, three remineralizing agents (nano-hydroxyapatite (n-HAP), casein phospho-peptide amorphous calcium phosphate (CPP-ACP), and bioactive glass (BAG)) were applied to the labial surface with traditional and with electrophoresis-aided techniques according to manufacture instructions. Surface topography and chemical composition were evaluated using ESEM/EDX at baseline, after demineralization, and after remineralization at different periods: (two weeks, five weeks, for the traditional technique, and three hours, five hours for the electrophoresis-aided technique). ESEM images were imported into Image J.software for further analysis. Results: P ≤ 0.05 was used to compare different variables using the ANOVA test and Tukey's post-hoc analysis. ESEM and image J. analysis showed that the effects of each of the three remineralizing agents enhanced the remineralization of surface topography of the enamel specimens following the different application periods. EDX results revealed that for the traditional technique; n-HAP showed the highest remineralizing effect followed by BAG with the lowest effect observed in the CPP-ACP material after two weeks. While after five weeks, n-HAP material revealed the highest remineralizing effect followed by CPP-ACP material with the lowest effect revealed in BAG material. Moreover, for the electrophoresis-aided technique; n-HAP material revealed the highest remineralizing effect, followed by CPP-ACP material with the most insignificant effect shown in BAG material after both three and five hours. Conclusions: The application of different remineralizing agents enhances enamel remineralization and has an influential effect on enamel surface topography and mineral composition. Further time has a significant positive impact on remineralization. Moreover, the dynamics of remineralizing agents might be efficiently accelerated by electrophoresis, amplifying their effects on demineralized enamel.

Keywords : Division: Meeting: 2023 Egyptian Section Meeting (Cairo, Egypt) Kamal Elmobasher

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Research Title :In vivo evaluation of electrophoresis-aided re-mineralization on surface topography and chemical composition of de-mineralized enamel: an in vivo animal model study

Authors :Kamal Eldeen Ahmed Elmobasher1 , Sameh Mahmoud Nabih2 , Hamed Ibrahim Mohamed

Abstract :Introduction: The most mineralized and rigid tissue in the human body is tooth enamel. Enamel, the outermost covering of teeth, is made of hydroxyapatite, which is arranged in a well-organized prism structure to absorb loads. De-mineralization occurs when the most widely available and soluble material is removed from the enamel rods’ periphery and it is exposed to acids, increasing the enamel’s surface porosity, and allowing more acids to diffuse into the tissue and lose mineral ions.Objectives: To evaluate whether an electrophoresis-aided system improved re-mineralization of de-mineralized enamel more than a traditional technique in rabbits with initial enamel lesions using different re-mineralizing agents. Material and methods: Initial enamel lesion was produced through acid etching, and three re-mineralizing agents, such as nano-hydroxyapatite, casein phospho-peptide amorphous calcium phosphate, and bioactive glass, were applied to labial surface using traditional or electrophoresis-aided techniques. Surface topography and chemical composition were evaluated using environmental scanning electron microscope/energy dispersive X-ray spectrometer at baseline, after de-mineralization, and after re-mineralization, at different time periods, such as 2 weeks and 5 weeks for a traditional technique, and 3 hours and 5 hours for an electrophoresis-aided technique. Results: Environmental scanning electron microscope showed that re-mineralizing agents enhanced re-mineralization of surface topography following different application periods. Energy dispersive X-ray spectrometer results revealed that for the traditional technique, there was no statistically significant difference between mean Ca : P ratio values of the three materials at 2 and 5 weeks. For the electrophoresis-aided technique, nanohydroxyapatite showed the highest effect, with the most insignificant effect demonstrated in bioactive glass at both 3 and 5 hours. Conclusions: Applying different re-mineralizing agents significantly influences enamel topography and chemical composition, and accelerate the effect of re-mineralizing agents. Moreover, the dynamics of re-mineralizing agents might be accelerated by electrophoresi

Keywords :electrophoresis, enamel re-mineralization, ESEM/EDX

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Research Title :Effect of electrophoresis on correlation between mineral content and enamel micro-hardness: a study in New Zealand rabbits

Authors :Kamal Eldeen Ahmed Elmobher Kamal Eldeen, Sameh Mahmoud Nabeih, Hamed Mahmoud Elkady

Abstract :Introduction: This in-vivo animal study investigated the correlation between calcium-to-phosphorus (Ca : P) ratio and enamel micro-hardness, and compared traditional re-mineralization techniques with electrophoresisaided techniques using different re-mineralizing agents. Objectives: The null hypothesis of the study was that there is a strong connection between Ca : P ratio and microhardness, and electrophoresis technique has a positive effect on the correlation between Ca : P ratio and enamel micro-hardness. Material and methods: In New Zealand rabbit models, initial enamel lesion was produced, followed by acid etching treated with three re-mineralizing agents: nano-hydroxyapatite (n-HAP), casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), and bio-active glass (BAG). Samples were subjected to de-mineralization and re-mineralization processes, with chemical composition and micro-hardness assessed with energy-dispersive X-ray analysis (EDX) and Vickers hardness testing, respectively. Measurements were obtained at baseline, post-de-mineralization, and post-re-mineralization at different time periods: two weeks and five weeks for traditional technique, and three hours and five hours for electrophoresis-aided technique. Results: Energy dispersive X-ray spectrometer (EDX) and Vickers micro-hardness tester results revealed significant differences in Ca : P ratios and micro-hardness in re-mineralizing agents. N-HAP exhibited the highest Ca : P ratio, while N-HAP and CPP-ACP showed superior micro-hardness compared with BAG. However, no significant correlation was identified between Ca : P ratio and micro-hardness. Electrophoresis accelerated re-mineralization kinetics, but did not enhance the relationship between mineral content and enamel micro-hardness. Conclusions: The findings highlight the efficacy of electrophoresis in promoting faster re-mineralization, while questioning its impact on the fundamental link between enamel composition and mechanical properties.

Keywords : correlation, micro-hardness, electrophoresis, mineral content, re-mineralization.

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