رضوى طاهر الشرقاوي

ASUجامعة العلوم التطبيقية الخاصة - الصفحة الرئيسيةكلية طب الأسنانرضوى طاهر الشرقاويالأبحاث

الأبحاث

Histo-pathological Evaluation of the Effect of Aloe Vera Gel as a Pulpotomy Agent in Young Permanent Teeth: A pilot Study

Authors :Ahmed Adel Salama, Rania A. Nasr and Radwa T. Elsharkawy

Abstract :Introduction: Preservation of pulp vitality is the principal objective in the management of exposed vital pulp of immature young permanent teeth. It has become challenging to identify the ideal pulp capping agent for each clinical case. Owing to the reported clinical and radiographic success of Aloe vera as a pulp capping material in primary teeth, it was tempting to investigate this material as a pulp medicament in young permanent teeth. Materials & Methods: Eight intact newly erupting immature young premolars, scheduled for extraction for orthodontic purposes, were selected for this pilot study. Two groups were implemented in the study; group I was applied Aloe vera gel as the pulpotomy material while group II received Portland Cement. Results: Histological sections of both studied groups showed well organized odontoblast-like cells with proximal nuclei and extended odontoblastic processes arranged near the orifices and along the peripheries of the radicular walls, accompanied by predentin layer and dentin with the classic arrangement of dentinal tubules. The gene expressions of IL-1β, TGF-β and DSPP were comparable between the Aloe vera and Portland cement group Conclusion: Based on the results of the current pilot study, Aloe vera gel proved comparable to the control group as a pulp capping material in pulpotomy of young permanent teeth

Keywords :Aloe Vera, Natural, Medicinal Plant, Young Permanent Teeth, Pulpotomy, Portland Cement, IL-1β, TGF-β, DSPP

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Effect of Vitamin C Administration 24 Hours After 5-Fluorouracil Versus its Concomitant Administration on Cellular Proliferation, Cell Death and Lipid Peroxidation in WI-38 Human Fibroblast Cell Line

Authors : Raneem F. Obeid , Radwa T. El-Sharkawy and Hala El-Kammar

Abstract :Background: 5-Fluorouracil (5-FU) is a routine anticancer agent for several cancer types, its short half-life requires continuous administration in high doses. This results in toxic side-effects to the normal tissues in the body. Vitamin C is an antioxidant which believed to impart cyto-protection to normal tissues in cancer patients treated with chemotherapeutic drugs. Aim of the Study: To assess the potential benefit of vitamin C administration 24 hours after 5-FU treatment compared to its immediate supplementation on normal fibroblast cell line. Materials and Methods: Fibroblast cell line (WI-38) was divided into 5 groups. Group I not treated (control group), group II treated with 5-FU only and group III received vitamin C only. Groups IV and V received 5-FU with vitamin C simultaneously and after 24 hours of 5-FU administration, respectively. The viability of cells in all groups was determined using MTT assay. MDA Quantification Assay was used to determine lipid peroxidation. Flow cytometry was used for evaluation of the cell cycle and cell death. All recorded data was statistically analyzed. Results: Cells in group IV seems like group II fewer and rounded while in group V some of them adherent spindle and the others rounded non-adherent. The least viability was noted in group II. MDA levels were significantly lower in groups III compared to all the other groups. Cell cycle analysis revealed the presence of statistically significant differences between groups in different phases of the cycle. Flowcytometry analysis showed the highest apoptosis in groups II and IV. Conclusion: Co-treatment of normal fibroblasts with vitamin C along with 5-FU reduces cell death and lipid peroxidation while enhancing viability and proliferation in treated fibroblasts, indicating healing and repair. Vitamin C administration 24 hours after 5-FU is more effective in reducing cell death and lipid peroxidation compared to its concomitant administration with 5-FU

Keywords : 5 fluorouracil, fibroblast cell line, lipid peroxidation, ROS, vitamin C

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The structure of cemento-dentinal junction in mandibular deciduous molar in Egyptian patients (Scanning electron microscope study)

Authors : Raneem F. Obeid and Radwa T. El-Sharkawy

Abstract :Introduction: Cementodentinal junction (CDJ) is the interface between cementum and dentin. The cause of this attachment was described by the intermingling of cemental and dentinal fibers or the accumulation of adhesive proteoglycans at the cementodentinal junction. Cementum was classified to three major types according to the type of fibers; Acellular extrinsic fiber cementum, Cellular intrinsic fiber cementum and Cellular mixed stratified cementum. Aim of Work: To observe the cemento-dentinal junction in human mandibular deciduous 2nd molar and mandibular permanent 1st molar by scanning electron microscopy combined by NaOH maceration. Materials and Methods: Twenty extracted mandibular deciduous 2nd molar and mandibular permanent 1st molar with complete root(s), were collected. The teeth preserved in 10% formalin then divided into two groups consisted of 10 teeth each. All teeth were cut mesiodistally into halves with a diamond desk, then the sectioned halves were demineralized by formic acid 10% for one month then were immersed in 10% NaOH aqueous solution for 2-3 days at room temperature and rinsed in distilled water overnight (24 hours). Results: Acellular cementum in mandibular deciduous 2nd molars showed a gap with complete separation, while in cellular cementum there is fibril poor gap and fibril intermingling between the intrinsic fibers of cellular cementum and dentin root fibers. The gap and the intermingling fibers were not observed in the examination of cellular cementum in mandibular permanent 1st molars while in acellular cementum there is a fiber intermingling observed. Conclusion: Fibril intermingling plays a role in cementum to dentin attachment

Keywords :Cementodentinal junction, deciduous molar, fiber intermingling, NaOH maceration, permanent molar

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Flowable Platelet Rich Fibrin A New Trend For Enhancing Bone Augmentation Results In Maxillary Sinus Floor Augmentation.(Radiographic And Histologic Evaluation)

Authors :Hesham S. Abdelmoneim and Radwa T. Elsharkawy

Abstract :Flowable PRF enriched and vibrant bioactivity, simplified preparation, high healing potentials and easy incorporation with other biomaterials were the key aspects to stimulate the applications of flowable PRF in various medical fields. The current study aimed to evaluate if flowable PRF has superior bone augmentation properties over the conventional PRF in sinus floor augmentation procedures. In this study 18 maxillary sinuses were augmented, which were divided into 2 groups, group A maxillary sinuses were augmented using a mixture of xenograft bone substitute, group B maxillary sinuses were augmented using a mixture of xenograft bone substitute and flowable PRF. Results: Clinically flowable PRF showed ease of preparation and improved the handling and manipulation of the bone graft particles. Radiographic results showed that Group A average gained bone height of 10.4±1.2 mms while in group B mean gained bone height was 11.6±1.9 mms, bone densities results of both groups showed that bone density units of group A were 578 ± 56 Hounsfield Units(HU), while group B calculated augmented bone densities were 586.2 ± 68 HU. Histologic evaluation stated that group I and group II revealed a lot of similarities regarding the amount and the quality of the newly formed bone. There by concluding that flowable PRF resulted in similar results to that of the conventional PRF, however the ease of preparation, application and manipulation emphasizes its benefits and recommends its application

Keywords : Flowable PRF, injectable PRF, sinus lifting, dental implants, PRF in maxillary sinus

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Histological evaluation of the ANTIOXIDANT effect of Vitamin E on reversing the negative impact of tartrazine on extraction socket healing. (Randomized controlled trial)

Authors : Raneem F. Obeid , Hesham S. Abdelmoneim and Radwa T. Elsharkawy

Abstract : One of a synthetic food dye which used worldwide is Tartrazine, it is an orange-colored, water soluble with a potential toxicological risk. Food or drinks containing tartrazine caused sensitivity symptoms. Vitamin E is an essential lipid-soluble vitamin and has well-established antioxidant properties which improve the proliferation, differentiation, and mineralization, and reduced apoptosis of osteoblastic cells. Aim of this study: to evaluate the effect of tartrazine and co-administration of vitamin E on post extraction socket using histological examination by light microscope. Materials and methods: Surgical extraction of mandibular first molars were done in a twenty one albino rats, the animals were randomly divided into 3 groups; group I (control), group II given tartrazine and group III given tartrazine and Vitamin E (treated group). The duration of the experiments was 14 days. Histological evaluation was done by H & E and Masson trichrome. Results: the bone healing of the extraction socket in the Vitamin E treated group was nearly the same as the control group. Conclusion: Vitamin E has a protective effect as an effective antioxidant on bone healing in post extraction socket and may be useful as a supportive agent in an endless array of dental surgeries

Keywords : Extraction, Socket, Vitamin E, Tartrazine, Antioxidant, food color, bone healing

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A 6-Month Histologic and Histomorphometric assessment of Xenograft or Xenograft combined with plasma rich in growth factors versus autogenous bone in sinus augmentation: A Randomized controlled trial

Authors :Rehab Tarek Elsharkawy , Nesreen Khairy and Radwa Taher Elsharkawy

Abstract :Purpose: The purpose of this study was to evaluate the effect of combining the PRGF to Xenograft on quality of the newly formed bone in open sinus lifting procedures after six months. Patients and methods: Thirty maxillary sinuses were augmented in twenty five patients, 18 males and 7 females, who had less than 5 mm of bone height. Maxillary sinuses were randomly allocated into one of three groups; the study group I, where the sinus was augmented using autogenous bone taken from the patient’s chin, group II, in which the sinus was augmented using Xenograft mixed with saline, group III, in which the sinus was augmented using Xenograft mixed with Platelets rich in growth factors (PRGF). After 6 months healing period, core biopsies were harvested and implant was simultaneously placed in the same site. Comparative microscopic and histomorphometric analysis were conducted and statistically analyzed. Implant loading was done after 6 months healing period. Results: After 6 months, the results of this study showed that there was no adverse effect noticed in any case during the follow-up period. Histological findings showed that the addition of PRGF promotes bone maturation preceding the bovine grafted material alone. The histological stained specimens of group III revealed a picture more or less resembling group I (autogenous bone). The amount of the residual graft particles in the group I and III were found to be less than group II. Conclusion: Combining PRGF to the xenograft improved the handling characteristics and the quality of the bone after 6 months in maxillary sinus augmentation procedure. Although PRGF combined to xenograft promoted bone maturation preceding the bovine grafted material alone, it did not prove to be superior to the autogenous bone which remains to be the gold standard for grafting

Keywords :Xenograft, Bovine bone, PRGF, Histomorphometric analysis, open sinus lift, maxillary sinus augmentation

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Evaluation of the Effect of Magnitude of Soft Tissues Surgical Trauma on Peri-implant Soft Tissues Stability. (Randomized control trial)

Authors : Hesham S. Abdel-Moneim, Radwa T. Elsharkawy and Ahmed Roshdy Radwan

Abstract :Peri-impant soft tissues stability became an increasingly thought after goal nowadays in almost every dental implant surgery especially in the esthetic zone, despite being totally over looked in the early stages of the oseointegration era. Implantologists previously focused mainly on achieving oseointegration and considered it to be the bench mark for dental implants success regardless to the esthetic outcomes. With the rise of esthetic dentistry and the use of computer guided technology in surgeries and restoration fabrication, peri implant soft tissues health and stability became of huge concern and a corner stone in dental implants overall success and survival longevity evaluation index. So this study aims to evaluate the effect of the minimally invasive implant surgeries versus the conventional flapped surgical procedures on the peri implant soft tissue around the dental implants. Promising results were postulated from the current study showing a marked superiority of health, speed of recovery, esthetics and stability of the peri implant soft tissues around dental implants placed by minimally invasive surgical protocols versus the conventional flapped surgical techniques. Thereby, concluding that minimally invasive surgical techniques results in an overall better dental implants outcomes aided by the advanced technology in both dental implants installations and final restorations

Keywords : Dental implants, peri-implant tissues, implant soft tissue esthetics, minimally invasive implant surgeries, implants soft tissue stability

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Potential Therapeutic Effect of Moringa Oleifera on Tongue papillae of Diabetic Albino Rats

Authors : Hala El-kammar, Raneem F. Obeid and Radwa T. Elsharkawy

Abstract :Background: The tongue is the second most commonly affected oral site after periodontal tissues in diabetes. Diabetic complications are mainly attributed to oxidative stress and side effects from long term use of drugs to treat diabetes. MO has been reported to be a valuable source of natural antioxidants. Materials and methods: this study comprised three groups; control, diabetic and MO treated groups. The experiment was terminated after fourteen days. Evaluation of the rats was done by measuring blood glucose levels, as well as, histological assessment of the tongue tissue by H&E and SEM. Results: There was a significant reduction in blood glucose levels and histological evaluation revealed improvement in the condition of the tongue mucosa in the MO treated group compared to the diabetic group. Conclusion: MO has a hypoglycemic effect on the blood glucose level of diabetic albino rats. MO may partially reverse some of the detrimental effects hyperglycemia has on the tongue mucosa of diabetic rats

Keywords :Diabetes, ROS, Moringa Oleifera, Alloxan, Tongue, Mucosa, Papilla, Hyperglycemia

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Effects of Moringa Oleifera Aqueous Leaf Extract on Submandibular Salivary Glands of Diabetic Albino Rats

Authors :Radwa T. El-sharkawy, Hala A. El-kammar, Raneem F. Obeid and Abdelfattah A. Bdelkhalek

Abstract :Moringa Oleifera (MO), also known as the ‘drumstick tree,’ is recognized as a nutritious and cheap source of phytochemicals, that a have a prominent anti-oxidative effect. Salivary gland dysfunction has been frequently reported in diabetic patients. Diabetes is a chronic metabolic disease that has complications mainly resulting from persistent hyperglycemia. Aim of the study: To Assess the effect of MO aqueous leaf extract on blood glucose levels in diabetic albino rats and its effect on submandibular salivary glands of diabetic albino rats. Materials and methods: the study comprised three groups; control, diabetic and MO treated groups. The experiment was terminated after fourteen days. The evaluation was performed by measuring the blood glucose levels and weight. Histological evaluation was done by H&E, PAS and IHC for COX-2. Results: the blood glucose levels and histological signs of diabetic complications were significantly lowered in the MO treated group. Conclusion: MO is a promising anti-diabetic treatment and may even reverse some of the diabetic complications

Keywords : COX-2, Diabetes, Moringa Oleifera, Reactive oxygen species, Submandibular salivary gland

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Effect of the parotid salivary gland on calcium and amylase enzyme levels in blood and its influence on bone healing in albino rats (Histological and radiographic study)

Authors : Medhat A. El-Zainy , Ahmed Mahmoud Halawa , Radwa Taher Elsharkawy

Abstract :The healing potential of bone is influenced by a variety of biochemical, cellular, hormonal and pathological mechanisms. As previous studies stated that parotid salivary glands may have endocrinal role, the aim of this study was to evaluate the effect of parotidectomy on bone healing and on calcium and amylase enzyme levels in blood. The rats were divided into two groups; control and experimental group. The control group was subjected to unilateral surgical mandibular defects, while the experimental group was subjected to the same procedure in addition to bilateral surgical removal of the parotid glands. Each of the control and experimental groups was further subdivided into 3 subgroups, A, B and C according to the time of termination corresponding to 4, 8 and 12 weeks respectively. Blood samples were obtained in order to determine calcium and amylase levels in blood. The surgically defected mandibles of each subgroup were analyzed postoperative to determine the radiographic bone density of the surgical defect throughout the healing process, then processed and examined histologically. Examination of the H & E stained sections of the mandibles at 4 weeks showed minimal bone formation from the defect margin of the experimental group in comparison with the control group. At 8 weeks, the experimental group showed increase of bone formation from the defect margin. At 12 weeks, the center of the defect was filled by a considerable amount of spongy bone and a definite reversal line between new and old bone. The Masson trichrome stained sections of the experimental group at 12 weeks presented a considerable amount of green collagen fibers. The average (mean) percentage of radiographic bone densities of the surgical defect of the experimental group slightly raised to 82.06 at 12 weeks. The serum amylase level at 4 weeks was less than the normal value then was slightly increased at 8 weeks and finally at 12 weeks increased more than the normal value. However, the serum calcium level was within the normal value in all experimental and control subgroups. It was concluded that bilateral parotidectomy in albino rats resulted in delayed bone healing and was associated with an initial drop in serum amylase level at 4 weeks, however serum amylase level was self-compensated at 8 and 12 weeks postoperatively, while it didn't significantly influence serum calcium level

Keywords :Bone healing Calcium Amylase enzyme Parotid salivary gland

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Effect of Nigella sativa on the integrity of parotid salivary gland of albino rats and its activity for insulin and glucagon. J Am Sci 2012; 8(12):1168-1172]

Authors : Samia M.Kamal ; RadwaT.ELsharkawy and Rehab A. Abdelmoneim

Abstract :This study investigated the effect of Nigella Sativa (N.sativa) on the histological features of parotid salivary glands of aging albino rats and its role on the activity of the glands for secretion of insulin- and glucagonlike peptides. Forty five male albino rats aged ten months were kept on the laboratory diet over a period of two months. Then, they were divided into three equal groups; young control (sacrificed at the end of the two months), old control (sacrificed three months later) and the experimental group (supplemented with N. sativa in a daily dose of 300mg/200gm body weight over a period of three months and then sacrificed). The parotid glands were then dissected out and subjected to histological and immunohistochemical investigations. The results showed only minimal amount of fibrosis and inflammatory cell infiltration in the N. sativa supplemented group. There were no distinctive changes in the architecture of the glands compared to that of young control. They did not show the prominent extensive features of aging manifested in the old aged control group. Moreover, the N. sativa supplemented group showed obvious increase in immunohistochemical reactivities for insulin and glucagon in the glandular tissue when compared to the rats of old control. Finally it could be concluded that N.sativa has got a cytoprotective effect against the degenerative changes of age and a beneficial role on the integrity of parotid salivary glands of aged rat. Also, N. sativa has been shown to increase the activity of parenchymal cells of rat parotid gland for insulin and glucagon that was markedly diminished with advance of age

Keywords : Nigella Sativa,parotid salivary gland, insulin, glucagon, immunohistochemistry, parenchymal cells

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